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open area  test


Testing: Movement and Anxiety Levels

parameter :

  1. Time spent on center and total area

  2. Distance traveled by center and total area

  3. speed in the central and surrounding areas

  4. Percentage of Time Spent in Central/Total - Anxiety Level

open field test

 

To test: locomotion and anxiety level

Parameters:

  1. time spent in central and total area

  2. distance moved in central and total area

  3. speed in central and peripheral area

  4. ratio of time spent in central/total area - anxiety level

Report Example: Central and Peripheral Area

Central and Peripheral Zone Tracking (pictured below)

Central Zone: (b) Blue Zone

zoneUnit: 'pixel (b)

'xData: [132 196 196 132 132]

yData : [79 79 164 164 79]

zone : [240x320 double]

frameRate: 10

nFrames: 713

RhoUnit: 'cm (3.2x4.25 pixels) or cm/sec'

totalRho : 308.3802

maxRho: 35.5210

meanRho: 4.3251

sdRho: 6.1961

seRho: 0.2320

Total Area: (c) Light Blue Area
​ zoneUnit: 'pixel (c)' 
xData: [100 228 228 100 100] 
yData: [36 36 206 206 36] 
zone: [240x320 double] 
frameRate: 10 
nFrames: 3001 
RhoUnit: 'cm (3.2x4.25 pixel) or cm/sec' 
totalRho: 1.8258e+003 
maxRho: 38.4496 
meanRho: 6.0841 
sdRho: 7.0839 
seRho: 0.1293

variable

in the total area,
1. Duration: 300.1 seconds
2. Total distance: 1825.8 cm
3. Speed (average): 6.0841 cm/sec


in the central area,
1. Duration: 71.3 seconds
2. Total distance: 308.3802 cm
3. Speed (average): 4.3251 cm/sec


4. Ratio of time spent in central/total zone: 0.23
 

Example Report: central and peripheral area

 

Tracking in central and peripheral area (Figure below)

Central area: (b)lue zone

zoneUnit: 'pixel (b)'

xData: [132 196 196 132 132]

yData: [79 79 164 164 79]

zone: [240x320 double]

frameRate: 10

nFrames: 713

RhoUnit: 'cm (3.2x4.25 pixel) or cm/sec'

totalRho: 308.3802

maxRho: 35.5210

meanRho: 4.3251

sdRho: 6.1961

seRho: 0.2320

Total area: (c)yan zone

zoneUnit: 'pixel (c)'

xData: [100 228 228 100 100]

yData: [36 36 206 206 36]

zone: [240x320 double]

frameRate: 10

nFrames: 3001

RhoUnit: 'cm (3.2x4.25 pixel) or cm/sec'

totalRho: 1.8258e+003

maxRho: 38.4496

meanRho: 6.0841

sdRho: 7.0839

seRho: 0.1293

Parameters

 

In total area,
1. Time spent: 300.1 sec
2. Total distance: 1825.8 cm
3. Speed (mean): 6.0841 cm/sec

In central area,
1. Time spent: 71.3 sec
2. Total distance: 308.3802 cm
3. Speed (mean): 4.3251 cm/sec

4. Ratio of time spent in central/total area: 0.23

Example report: defined zones

Tracking (picture below)

Time spent in each zone:

  • Blue Zone: 71.3 seconds

  • Sky blue  Zone: 83.2 seconds

  • Green Zone: 40.3 seconds

  • Black Zone: 74.0 seconds

  • Pink Zone: 30.6 seconds

Other parameters can also be calculated.

Example Report: defined zones

 

Tracking (Figure below)

Time spent in each zone:

  • blue zone: 71.3 sec

  • cyan zone: 83.2 sec

  • green zone: 40.3 sec

  • black zone: 74.0 sec

  • magenta zone: 30.6 sec

Other parameters can be also calculated.

Example report: 4x4 rectangular area

Multiple Zone Tracking (pictured below)

Time spent in each zone:
  28.6  12.2  16.1  35.4

  7.8  15.8  8.0  10.5

  47.0  53.0  3.8  7.8   

   21.7  4.1  2.8  25.5 

Example Report: 4x4 rectangle zones

tracking multiple zones (Figure below)

 

Time spent in each zone:
  28.6 12.2 16.1 35.4

  7.8 15.8 8.0 10.5

  47.0 53.0 3.8 7.8

  21.7 4.1 2.8 25.5

Example report: 20x20 rectangular area

Tracking in multiple zones (pictured below)

Time spent in each zone (table below)

Example Report: 20x20 rectangle zones

Tracking in multiple zones (Figure below)

Time spent in each zone (Table below)

reference

 

[Heredia et al., 2014] , assessing movement and anxiety in mice

Reference

 

[Heredia et al., 2014] , assess locomotion and anxiety in mice

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